You can find information on the following topics covering the process of protein expression and purification.
If you have additional links or comments please let us know.
Insect cell expression
- Purification Strategy: Handbooks, Technique selection guides, Columns, Buffers, Virus Purification, etc. PROTOCOLS
- Preparation of Cell Lysates / Different Disruption Methods. Protease Inhibitors. DNA removal. Endotoxin removal. Detergents PROTOCOLS
- Purification of Recombinant Proteins: poly His-Tagged, GST, MBP, Strep-tag, Calmodulin, Intein, CBD, SUMO, etc.PROTOCOLS
- Cleavage of Recombinant Proteins: Thrombin, Factor Xa, TEV-Protease, Enterokinase, SUMO, PreScission, TAGZyme, etc. Proteases sensitivity to Detergents. PROTOCOLS
- Conventional Chromatography: Gel Filtration, Ion Exchange, Hydrophobic Interaction, Multimodal, Hydroxyapatite, Reverse Phase Chromatography, Chromatofusing, Thiophilic.
- Affinity Chromatography: Activated Resins, Antibody Purification and antibody fragmentation, Lectins and Glycoprotein Purification, Deglycosylation, Immunoprecipitation. PROTOCOLS.
- Other Purification Procedures: Desalting, Ultrafiltration, Concentration, Dialysis, Protein Quantitation. PROTOCOLS.
- Protein Aggregation: Kits. PROTOCOLS
- Gel Electrophoresis: PAGE-SDS, Native gels, Gel Stain, Western, etc. PROTOCOLS
Protein Characterisation & Quality Control