Darren Hart


Institut de Biologie Structurale

AutomationProtein ProductionStructural VirologyDirected EvolutionProtein EngineeringThree-dimensional structural virologyinfluenza virusesHistone deacetylases
  • PhD 1996, Oxford University, UK.
  • Postdoctoral research at Cambridge University, UK.
  • Group Leader at Sense Proteomic Ltd., Cambridge, UK.
  • Team Leader at EMBL Grenoble 2003-2014.
  • CNRS Research Director, IBS Grenoble since 2014.
  • CNRS Unit Director (ISBG-Instruct 2)
ESPRIT: an automated, library-based method for mapping and soluble expression of protein domains from challenging targets. Yumerefendi H, Tarendeau F, Mas PJ, Hart DJ J. Struct. Biol. 2010 Oct; doi.org/10.1016/j.jsb.2010.02.021
ORF-selector ESPRIT: a second generation library screen for soluble protein expression employing precise open reading frame selection. An Y, Yumerefendi H, Mas PJ, Chesneau A, Hart DJ J. Struct. Biol. 2011 Aug; doi.org/10.1016/j.jsb.2011.04.004
Library-based methods for identification of soluble expression constructs. Yumerefendi H, Desravines DC, Hart DJ Methods 2011 Sep; doi.org/10.1016/j.ymeth.2011.06.007
Structure and inhibition of herpesvirus DNA packaging terminase nuclease domain. Nadal M, Mas PJ, Mas PJ, Blanco AG, Arnan C, Solà M, Hart DJ, Coll M Proc. Natl. Acad. Sci. U.S.A. 2010 Sep; doi.org/10.1073/pnas.1007144107
Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit. Tarendeau F, Boudet J, Guilligay D, Mas PJ, Bougault CM, Boulo S, Baudin F, Ruigrok RW, Daigle N, Ellenberg J, Cusack S, Simorre JP, Hart DJ Nat. Struct. Mol. Biol. 2007 Mar; www.ncbi.nlm.nih.gov/pubmed/17310249
Influenza Polymerase Can Adopt an Alternative Configuration Involving a Radical Repacking of PB2 Domains. Thierry E, Guilligay D, Kosinski J, Bock T, Gaudon S, Round A, Pflug A, Hengrung N, El Omari K, Baudin F, Hart DJ, Beck M, Cusack S Mol. Cell 2016 Jan; doi.org/10.1016/j.molcel.2015.11.016
Secondary Structure Prediction of Protein Constructs Using Random Incremental Truncation and Vacuum-Ultraviolet CD Spectroscopy. Pukáncsik M, Orbán Á, Nagy K, Matsuo K, Gekko K, Maurin D, Hart D, Kézsmárki I, Vertessy BG PLoS ONE 2016 Jan; doi.org/10.1371/journal.pone.0156238
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Simon B, Huart AS, Temmerman K, Vahokoski J, Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M Structure 2016 Jun; doi.org/10.1016/j.str.2016.03.020
Library methods for structural biology of challenging proteins and their complexes. Hart DJ, Waldo GS Curr. Opin. Struct. Biol. 2013 Jun; doi.org/10.1016/j.sbi.2013.03.004
Interaction of the influenza A virus polymerase PB2 C-terminal region with importin alpha isoforms provides insights into host adaptation and polymerase assembly. Boivin S, Hart DJ J. Biol. Chem. 2011 Mar; doi.org/10.1074/jbc.M110.182964
Structural Basis for the Oligomerization of the MADS Domain Transcription Factor SEPALLATA3 in Arabidopsis. Puranik S, Acajjaoui S, Conn S, Costa L, Conn V, Vial A, Marcellin R, Melzer R, Brown E, Hart D, Theißen G, Silva CS, Parcy F, Dumas R, Nanao M, Zubieta C Plant Cell 2014 Sep; www.ncbi.nlm.nih.gov/pubmed/25228343