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The Biacore technology allows real-time detection and monitoring of biomolecular binding events. One of the interacting molecules (the ligand) is bound to the biosensor surface (sensor chip), whereas the other is delivered to the surface in a continuous buffer flow through a microfluidic system. Binding to the immobilized molecule is followed by surface plasmon resonance, which detects mass changes at the sensor surface. Recording SPR signal variation as a function of time (sensorgram) for several analyte concentrations allows to determine the association and dissociation rate constants, and to derive the affinity constant. This technology also allows to measure the concentration of functional molecules and the interaction stoichiometry.
The Biacore technology allows real-time detection and monitoring of biomolecular binding events. One of the interacting molecules (the ligand) is bound to the biosensor surface (sensor chip), whereas the other is delivered to the surface in a continuous buffer flow through a microfluidic system. Binding to the immobilized molecule is followed by surface plasmon resonance, which detects mass changes at the sensor surface. Recording SPR signal variation as a function of time (sensorgram) for several analyte concentrations allows to determine the association and dissociation rate constants, and to derive the affinity constant. This technology also allows to measure the concentration of functional molecules and the interaction stoichiometry.
Visit the Grenoble ISBG/SPR platform webpage for more information on access and costs :
http://www.isbg.fr/biophysics-characterisation/spr-biacore-technology/
