Service / Technology Instance

About Protein expression in insect cells - NKI

View All Baculovirus Expression at Instruct

Depending on the project, the platform for expression will be chosen. Proteins can be expressed in E. coli, Insect cells and mammalian cells. For each of the systems we can do small scale (few ml) as well as large scale (few liters) production. For insect cell expression SF9 and SF21 cells are used for expression of intracellular and extracellular proteins. The cells are grown in suspension cultures in volumes up to 500 ml/flask. We use Baculovirus technology for over-expression of proteins in insect cells.

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Depending on the project, the platform for expression will be chosen. Proteins can be expressed in E. coli, Insect cells and mammalian cells. For each of the systems we can do small scale (few ml) as well as large scale (few liters) production. For insect cell expression SF9 and SF21 cells are used for expression of intracellular and extracellular proteins. The cells are grown in suspension cultures in volumes up to 500 ml/flask. We use Baculovirus technology for over-expression of proteins in insect cells.

User Guide

Please see the NKI protein facility website for more information.

Instruct Centre

Protein facility - NKI

NKI Protein Facility

Plesmanlaan 121

1066CX

Amsterdam

Netherlands

www.nki.nl/topmenu/protein-facility/

Contacts:

Anastassis Perrakis
Anastassis Perrakis
The Netherlands Cancer Institute
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Reinout Raijmakers
Reinout Raijmakers
Bijvoet Center, Utrecht University
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Patrick Celie
Patrick Celie
The Netherlands Cancer Institute
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