Service / Technology Instance

About Electron Microscopy, Strasbourg, France

The center offers access for three-dimensional structure determination using cryo-electron microscopy (cryo-EM). The centre has expertise in all aspects of single particle analysis from the automated acquisition of large data sets to image analysis. 

Service Availability:

Remote

Physical

Instruments Available:

The center offers access for three-dimensional structure determination using cryo-electron microscopy (cryo-EM).The centre has expertise in all aspects of single particle analysis from the automated acquisition of large data sets to image analysis . We offer access to the the Glacios cryo-electron microscope which allows high-resolution SPA and CET automated data collection. Its FEG is usually tuned to 200keV. An automatic loading mechanism allows mounting twelve grids at a time. This microscope is equipped with two digital cameras: FEI Ceta-D and the direct electron detector GATAN K2

The center offers access for three-dimensional structure determination using cryo-electron microscopy (cryo-EM).The centre has expertise in all aspects of single particle analysis from the automated acquisition of large data sets to image analysis . Access to the following instrumentation is provided: The Tecnai F20 is equipped with a FEG operated at 100keV or 200keV.

The center offers access for three-dimensional structure determination using cryo-electron microscopy (cryo-EM). The centre has expertise in all aspects of single particle analysis from the automated acquisition of large data sets to image analysis . The Titan Krios cryo electron microscope is the latest generation electron microscope with capacities for high-resolution data collection for both Cryo Electron Tomography (CET) experiments and Single Particle Analysis (SPA) with automated data collection. The electron source is a Field Emission Gun (FEG) that can be operated at 80keV, 100keV, 200keV or 300keV. An automatic loading mechanism allows mounting twelve grids at a time.

The Titan 1 electron microscope is equipped with CMOS (FEI FALCON3 4K*4K and GATAN K3) high-sensitivity direct electron detector cameras, Cs corrector, phase plate and GIF.

The Titan 2 (G4) is equipped with Selectris X energy filters and Falcon 4i camera.

User Guide

Access to the following instrumentation is provided: the Glacios cryo-electron microscope which allows high-resolution SPA and CET automated data collection and two Titan Krios cryo-electron microscopes with CMOS high-sensitivity direct electron detector camera. The Tecnai F20 is equipped with a FEG operated at 100keV or 200keV.

Titan Krios cryo electron microscopes

The Titan Krios is the latest generation electron microscope with capacities for high-resolution data collection for both Cryo Electron Tomography (CET) experiments and Single Particle Analysis (SPA) with automated data collection. The electron source is a Field Emission Gun (FEG) that can be operated at 80keV, 100keV, 200keV or 300keV. An automatic loading mechanism allows mounting twelve grids at a time.

The Titan 1 microscope is equipped with CMOS (FEI FALCON3 4K*4K and GATAN K3) high-sensitivity direct electron detector cameras, corrector CS, phase plate and GIF.

The Titan 2  microscope (G4) is equipped with Selectris X energy filters and Falcon 4i camera.

The Glacios cryo electron microscope

The Glacios cryo-electron microscope allows high-resolution SPA and CET automated data collection. Its FEG is usually tuned to 200keV. An automatic loading mechanism allows mounting twelve grids at a time. This microscope is equipped with two digital cameras: FEI Ceta-D and the direct electron detector GATAN K2

The Tecnai F20

The Tecnai F20 is equipped with a FEG operated at 100keV or 200keV. Grids are mounted one at a time using a Gatan side-entry cryo-holder. This microscope is equipped with one digital camera (GATAN CCD 2K*2K "US10001"), it is used to collect data for cryo-SPA or room temperature electron tomography using a Fischion side-entry holder. Cryo-EM grids are flash-frozen in a temperature and humidity controlled environment using a Vitrobot system (FEI).

All the microscopes are equipped with digital cameras, images can be recorded as films and scan them with a high-resolution Heidelberger Druckmaschinen drum scanner (5 micron pixel size). When asking access for Titan or Glacios, please add to your proposal preliminary image of the sample in cryo condition.

Instruct Centre

Instruct Centre FR1

IGBMC

1, rue Laurent Fries

Illkirch

Strasbourg

France

www.igbmc.fr

Electron Microscopy, Strasbourg, France

Contacts:

Marie-Christine Poterszman
Marie-Christine Poterszman
IGBMC-CERBM
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Corinne Crucifix
Corinne Crucifix
IGBMC-CERBM
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Alexandre Durand
Alexandre Durand
Inserm
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nils marechal
nils marechal
CNRS Délégation Alsace
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