Service / Technology Instance

About Electron Microscopy, NeCEN, Leiden, The Netherlands

NeCEN is an open access facility for high resolution cryo-electron microscopy of biological samples. Two state-of-the-art Titan Krios transmission electron microscopes allow efficient and automated high resolution data collection in its two most broadly used forms, single particle analysis (SPA) and cryo-electron tomography (cryo-ET). These imaging techniques allow visualisation of biomolecular structures, such as proteins, macromolecular complexes, bacteria, and cell organelles at subnanometer resolution in close-to-native conditions (no chemical fixation or staining required).

Instruments Available:

Titan Krios 1 is a dedicated instrument for EFTEM (energy-filtered TEM) and cryo-tomography. This microscope has a SFEG source and is equipped with a Gatan GIF+K2 DED (integrated into EPU allowing auto data collection) as well as a FEI Falcon 2 DED camera and volta phase plate. It is an instrument for both tomography and automated single particle acquisition.

Titan 2 is a specialised instrument for single particle acquisition. This microscope has a XFEG source, a Cs corrector and a FEI Falcon 3 counting mode DED camera. It is a dedicated instrument for automated single particle acquisition.

User Guide

Titan Krios 1 is a dedicated instrument for energy-filtered TEM (EFTEM) and cryo-electron tomography. This microscope has a S-FEG source and is equipped with a Gatan GIF+K2 DED as well as a volta phase plate. It is an instrument for both tomography and automated single particle acquisition.

Titan Krios 2 is a specialised instrument for single particle acquisition. This microscope has a X-FEG source, a Cs corrector, a Falcon 3 counting mode DED camera and volta phase plate.

Instruct Centre

NeCEN - LU

NeCEN

Gorlaeus Laboratory - Einsteinweg 55

2333 CC, Leiden

Netherlands

www.necen.nl

Electron Microscopy, NeCEN, Leiden, The Netherlands

Contacts:

Ludo Renault
Ludo Renault
Leiden University
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