Cell free expression landing text
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Cell-free expression is in particular suitable for the production of difficult proteins such as membrane proteins, toxins, complexes or peptides. Expression is performed in cell-free extracts of different origins and reactions can be supplemented with a variety of compounds such as ligands, cofactors, inhibitors, chemicals, detergents or lipids. Reaction volumes are small in the range of few ml and incubation times usually do not exceed 12-16 hrs. With optimized production protocols, several mg of protein in one ml of reaction can be obtained.