Bacterial expression

Production of recombinant proteins and protein complexes in bacterial hosts

 
Ray Owens Ray Owens University of Oxford email hidden
Anastassis Perrakis Anastassis Perrakis The Netherlands Cancer Institute email hidden

Instruct has 6 centres offering Bacterial expression across Europe. Navigate the map and click on the pins to discover centres near you.

Bacterial expression Details

Bacterial hosts are hosts of choice for the production of recombinant proteins and protein complexes. This is largely due to the ease of handling and genetically modifying these hosts. In addition, the short doubling time of bacteria enables fast production of samples using parallelised and robotised approaches. Furthermore, the large biomass and high expression yields that can be obtained with bacterial hosts generally enables the production of sufficient amounts of samples for structural studies.

User Guide

The main steps for bacterial expression include the construction of expression vectors, the transformation of the bacterial host with these vectors, the expression of the samples in the host, and the purification of the samples.

Several different cloning techniques are currently available that enable easy expression vector construction and that are compatible with parallelized and robotized strategies.

Sample expression range from expression of single proteins to the multi-expression of protein complexes. Various sets of expression vectors are available at several Instruct centers that enable sample expression to be carried out.