Advanced methods for the integration of other structural data with NMR data

Training
Date: 21-Jan-2013 1:00 CET to -

Contact: Antonio Rosato

Instruct

An Instruct funded course

Course overview:

The course aims at training young researchers in the combined use of data from various structural techniques, such as SAXS, X-ray diffraction or EM, together with NMR data to achieve a deeper understanding of the structural properties of macromolecular systems, from individual proteins to macromolecular adducts. Various methods based on the integration of NMR and SAXS data for the study of the dynamics of multidomain proteins will also be demonstrated.

Outline of course contents:

Lectures

  • Integration of SAXS and NMR data for the analysis of protein structure/dynamics
  • Modeling of large multi-molecular complexes by integration of X-ray-crystallography, NMR, mass spectrometry and electron microscopy data
  • Data-driven protein docking
  • Automation in NMR data analysis
  • Molecular dynamics for NMR-based protein structure refinement
  • Data-driven structure assessment.

Hands-on practicals(can be adjusted also depending on the availability of data from the attendees)

  • The AMPS-NMR portal for structure refinement
  • The HADDOCK portal for data-driven docking
  • The MAX-OCC portal for domain dynamics in multidomain proteins
  • Modeling of large multi-molecular complexes by integration of X-ray-crystallography, NMR, mass spectrometry and electron microscopy data
  • Protein structure validation.

Case studies will be provided via the web for re-examination also at the home lab.


Lecturers and Tutors

  • A.M. Bonvin (University of Utrecht)
  • G. Parigi (University of Florence)
  • A. Rosato (University of Florence)
  • H.J. Wolfson (Tel Aviv University)
  • L. Ferella (University of Florence)
  • E. Ravera (University of Florence)

Applications:

In order to send your application: please, complete the application form here.

Deadline for application: October 31st, 2012

Registration fee:

The registration fee for selected participants is € 275; the registration fee is waived for attendees from countries that formally joined INSTRUCT.

The fee includes lunches and coffee breaks during the course, plus the social dinner.

INSTRUCT is providing support to attendees from countries that formally joined INSTRUCT