Instruct Centre in the field of large scale virus production and development of virus purification methods for structural analysis (ICVIR)
Our platform provides large amounts of pure viruses and macromolecular complexes for structural studies using traditional ultracentrifugation methods. The platform is equipped with seven ultra and four superspeed centrifuges as well as gradient making and fractionation instrumentation. We also develop new methods for virus purification using asymmetric flow field flow fractionation (AF4) and monolithic chromatography in co-operation with the manufacturers (Postnova and Biaseparations). We also develop novel bioinformatics tools for comparison of protein sequences and structures. Our technologies are available for external users. They can be used in different combinations to purify large macromolecular complexes, such as viruses.
Instruct Centre in the field of large scale virus production and development of virus purification methods for structural analysis (ICVIR).
ICVIR is the only Instruct Centre in Nordic countries. It provides methods and principles developed for the production and purification of virus-sized particles from simple to highly complex structures and masses in the order of tens to hundreds of MDa's. As viruses are the most abundant life forms and causative agents of severe infectious diseases in all cellular organisms, it is important to include them in the Instruct activities and to improve the methods to produce and purify viruses for structural studies.
Our platform is supported by the expertise on the following fields:
For information about our research done under the Programme on Molecular Virology at the University of Helsinki, please visit:http://www.helsinki.fi/molecularvirology/.
Instruct Centre ICVIR is located at the Biocenter of the University of Helsinki, Helsinki, Finland. This Biocenter is situated at the Viikki Campus ~10 km from the city center and is easily accessible by public transport in ~30 minutes. More information on the Viikki Campus and the busses and their time tables can be found from the University of Helsinki web pages (http://www.helsinki.fi/viikki/english/). The Helsinki Vantaa International airport is located ~20 min drive from the Viikki Campus.
Viikki Campus hosts four faculties (Faculty of Biological and Environmental Sciences, Faculty of Pharmacy, Faculty of Agriculture and Forestry and Faculty of Veterinary Medicine), two research institutes of the University of Helsinki (Institute of Biotechnology and Neuroscience Centre) and the Viikki Science Library.
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